Benjamin Cravatt

Jan 11, 2025 | biorxiv.org | Charneal Dixon |Noah Martin |Micah J. Niphakis |Benjamin Cravatt

AbstractBACKGROUND AND AIMS: NOD2 is an intracellular innate immune receptor that detects bacterial peptidoglycan fragments. Although nominally soluble, some NOD2 is associated with the plasma membrane and endosomal compartments for microbial surveillance. This membrane targeting is achieved through post-translational S-acylation of NOD2 by the protein acyltransferase ZDHHC5. Membrane attachment is necessary to initiate a signaling cascade in response to cytosolic peptidoglycan fragments.

Nov 26, 2024 | cell.com | Kevin Scott |Hiroyuki Kojima |Nathalie Ropek |Charles Warren |Tiffany Zhang |Simon Hogg | +9 more

Keywordscovalent immunomodulatorsactivity-based protein profilinginterleukin-2-inducible T-cell kinasealternative splicingsplicing factor 3b subunit 1Get full text accessLog in, subscribe or purchase for full access. References1. Lynch, K.W.Consequences of regulated pre-mRNA splicing in the immune systemNat. Rev. Immunol. 2004; 4:931-9402. Martinez, N.M. ∙ Pan, Q. ∙ Cole, B.S. ... Alternative splicing networks regulated by signaling in human T cellsRNA. 2012; 18:1029-10403. Pan, Q. ∙ Shai, O.

Nov 6, 2024 | nature.com | L. T. Hung |Britney Jiayu He |Jens Luebeck |Lotte Brückner |Xiaowei Yan |Rocio Gonzalez | +8 more

AbstractThe chromosomal theory of inheritance dictates that genes on the same chromosome segregate together while genes on different chromosomes assort independently1. Extrachromosomal DNAs (ecDNAs) are common in cancer and drive oncogene amplification, dysregulated gene expression and intratumoural heterogeneity through random segregation during cell division2,3. Distinct ecDNA sequences, termed ecDNA species, can co-exist to facilitate intermolecular cooperation in cancer cells4.

Oct 15, 2024 | cell.com | Benjamin Cravatt |Michael Erb

Keywords FOXA1 covalent proteomics activity-based protein profiling cysteine chromatin pioneer transcription factor ATAC-seq ChIP-seq Introduction Lineage-specific transcription is controlled by enhancers harboring transcription factor (TF)-binding sites.1 Most TFs, however, cannot associate with condensed (e.g., nucleosome-bound) DNA and therefore rely on pioneer TFs to first bind to and open such heterochromatin sites in a locus-specific manner.2 Due to their first-order control of enhancer...

Jul 20, 2024 | nature.com | Gloria Asantewaa |Nathan P. Ward |Yun Pyo Kang |Yumi Kim |Nomeda Girnius |Fabio Hecht | +11 more

AbstractCells rely on antioxidants to survive. The most abundant antioxidant is glutathione (GSH). The synthesis of GSH is non-redundantly controlled by the glutamate-cysteine ligase catalytic subunit (GCLC). GSH imbalance is implicated in many diseases, but the requirement for GSH in adult tissues is unclear. To interrogate this, we have developed a series of in vivo models to induce Gclc deletion in adult animals. We find that GSH is essential to lipid abundance in vivo.