James M. Fulcher

Dec 4, 2024 | nature.com | James M. Fulcher |Hugh Mitchell |David Degnan |Lisa M. Bramer |Ronald Moore |Wei-Jun Qian | +1 more

AbstractSingle-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), an integrated platform that enables global profiling of the transcriptome and proteome from same single cells via RNA sequencing and mass spectrometry-based proteomics, respectively.

Jul 4, 2024 | biorxiv.org | James M. Fulcher |Hugh Mitchell |Sarah Williams |Kristin M Engbrecht

AbstractSingle-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), an integrated platform that enables global profiling of the transcriptome and proteome from same single cells using RNA sequencing and mass spectrometry-based proteomics, respectively.

Feb 13, 2024 | biorxiv.org | Kevin J. Zemaitis |James M. Fulcher |Rashmi Kumar |David Degnan

AbstractBackground: The Human Proteome Project has credibly detected nearly 93% of the roughly 20,000 proteins which are predicted by the human genome. However, the proteome is enigmatic, where alterations in amino acid sequences from polymorphisms and alternative splicing, errors in translation, and post-translational modifications result in a proteome depth estimated at several million unique proteoforms.