Wei-Jun Qian

Dec 4, 2024 | nature.com | James M. Fulcher |Hugh Mitchell |David Degnan |Lisa M. Bramer |Ronald Moore |Wei-Jun Qian | +1 more

AbstractSingle-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), an integrated platform that enables global profiling of the transcriptome and proteome from same single cells via RNA sequencing and mass spectrometry-based proteomics, respectively.

Nov 12, 2024 | nature.com | Masaji Sakaguchi |Kimi Araki |Wei-Jun Qian |Yu-Hua Tseng |Rohit Kulkarni |C. Ronald Kahn

AbstractLipodystrophy and obesity are associated with insulin resistance and metabolic syndrome accompanied by fat tissue dysregulation. Here, we show that serine protease inhibitor A1 (SerpinA1) expression in the liver is increased during recovery from lipodystrophy caused by the adipocyte-specific loss of insulin signaling in mice.

Mar 18, 2024 | nature.com | Qi Cui |Meg Shieh |Tony Pan |Akiyuki Nishimura |Tetsuro Matsunaga |Shane Kelly | +7 more

AbstractReactive sulfane sulfur species such as persulfides (RSSH) and H2S2 are important redox regulators and closely linked to H2S signaling. However, the study of these species is still challenging due to their instability, high reactivity, and the lack of suitable donors to produce them. Herein we report a unique compound, 2H-thiopyran-2-thione sulfine (TTS), which can specifically convert H2S to HSOH, and then to H2S2 in the presence of excess H2S.

Mar 1, 2024 | nature.com | Sanjay Jain |Liming Pei |Jeffrey M. Spraggins |Joana Gonçalves |John Hickey |Neil L. Kelleher | +9 more

Correction to: Nature Cell Biology https://doi.org/10.1038/s41556-023-01194-w. Published online 19 July 2023. In the version of this article originally published, the name of Tianyang Xu was misspelled as Tiangyang Xu. The name has been corrected in the HTML and PDF versions of the article. About this articleJain, S., Pei, L., Spraggins, J.M. et al. Author Correction: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP). Nat Cell Biol (2024).

Jul 19, 2023 | nature.com | Sanjay Jain |Liming Pei |Jeffrey M. Spraggins |Joana Gonçalves |John Hickey |Neil L. Kelleher | +9 more

Data types, organs and technologiesSince the second quarter of 2023, HuBMAP datasets using 18 different analytical technologies or assay types have been publicly available (Figs. 1a and 2). These encompass 1,672 publicly available datasets from regions with no histopathologic abnormality from 14 organs across 180 donors. Protocols were optimized to minimize the ischaemia time of healthy donors. All published data are freely available via the HuBMAP portal (https://portal.hubmapconsortium.org).