Mikhail M. Savitski

Jul 27, 2024 | biorxiv.org | Cecile Le Sueur |Magnus Rattray |Mikhail M. Savitski |EMBL Heidelberg

AbstractThermal proteome profiling (TPP) is a proteome wide technology that enables unbiased detection of protein drug interactions as well as changes in post-translational state of proteins between different biological conditions. Statistical analysis of temperature range TPP (TPP-TR) datasets relies on comparing protein melting curves, describing the amount of non-denatured proteins as a function of temperature, between different conditions (e.g. presence or absence of a drug).

Jun 8, 2024 | biorxiv.org | Mitsuhiro Matsuda |Henrik M. Hammarén |Jorge Lázaro |Mikhail M. Savitski

AbstractHuman embryonic development is generally slower compared with mouse, and one of the model systems for such inter-species differences in developmental tempo is the segmentation clock. The oscillation period of the human segmentation clock, as measured in induced presomitic mesoderm (iPSM) cells, is two times longer than that of mouse.

Oct 30, 2023 | biorxiv.org | Magnus Rattray |Mikhail M. Savitski |EMBL Heidelberg |Cecile Le Sueur

AbstractThermal proteome profiling (TPP) is a proteome wide technology that enables unbiased detection of protein drug interactions as well as changes in post-translational state of proteins between different biological conditions. Statistical analysis of temperature range TPP (TPP-TR) datasets relies on comparing protein melting curves, describing the amount of non-denatured proteins as a function of temperature, between different conditions (e.g. presence or absence of a drug).

Jun 26, 2023 | nature.com | Josy ter Beek |Laura Alvarez |André Mateus |Remy Colin |Athanasios Typas |Mikhail M. Savitski | +3 more

AbstractTo explore favourable niches while avoiding threats, many bacteria use a chemotaxis navigation system. Despite decades of studies on chemotaxis, most signals and sensory proteins are still unknown. Many bacterial species release d-amino acids to the environment; however, their function remains largely unrecognized. Here we reveal that d-arginine and d-lysine are chemotactic repellent signals for the cholera pathogen Vibrio cholerae.