Agnieszka Rybak-Wolf

Aug 22, 2024 | nature.com | Selene Lickfett |Agnieszka Rybak-Wolf |Carmen Menacho |Stephanie Le |Tancredi Massimo Pentimalli |Daniel Oehler | +14 more

AbstractExpansion of the glutamine tract (poly-Q) in the protein huntingtin (HTT) causes the neurodegenerative disorder Huntington’s disease (HD). Emerging evidence suggests that mutant HTT (mHTT) disrupts brain development. To gain mechanistic insights into the neurodevelopmental impact of human mHTT, we engineered male induced pluripotent stem cells to introduce a biallelic or monoallelic mutant 70Q expansion or to remove the poly-Q tract of HTT.

Sep 21, 2023 | nature.com | Ivano Legnini |Agnieszka Rybak-Wolf |Ricardo Wurmus |Anastasiya Boltengagen |Stefan Kempa |Andrew Woehler | +3 more

AbstractOrganoids derived from stem cells have become an increasingly important tool for studying human development and modeling disease. However, methods are still needed to control and study spatiotemporal patterns of gene expression in organoids. Here we combined optogenetics and gene perturbation technologies to activate or knock-down RNA of target genes in programmable spatiotemporal patterns.

Jun 22, 2023 | nature.com | Agnieszka Rybak-Wolf |Emanuel Wyler |Ivano Legnini |Seung Joon Kim |Benedikt B. Kaufer |Andrew Woehler | +2 more

Study designIn this study, we used human cerebral organoids as a model for acute HSV-1. The aim of the study was as follows: (1) to assess cellular and molecular signatures associated with viral infection; (2) to study the outcome of the current antiviral therapy used in clinical practice (ACV); (3) to identify and test better potential therapeutic approaches based on the results of 1 and 2.

May 17, 2023 | nature.com | Monika Piwecka |Agnieszka Rybak-Wolf

Authors and AffiliationsInstitute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, PolandMonika PiweckaBerlin Institute for Medical Systems Biology (BIMSB), Max Delbrueck Center for Molecular Medicine, Berlin, GermanyNikolaus Rajewsky & Agnieszka Rybak-WolfContributionsA.R.-W. and M.P. researched data for the article. A.R-W. and M.P. contributed substantially to discussion of the content. M.P. and A.R-F. wrote the article.