Andrej Sali

May 23, 2024 | nature.com | Luis Ferrández-Peral |Claudia Fontsere |Merve Ceylan |Giovanni Lara |Jacques Dainat |Arthur O. Zalevsky | +8 more

AbstractSLC22A10 is an orphan transporter with unknown substrates and function. The goal of this study is to elucidate its substrate specificity and functional characteristics. In contrast to orthologs from great apes, human SLC22A10, tagged with green fluorescent protein, is not expressed on the plasma membrane. Cells expressing great ape SLC22A10 orthologs exhibit significant accumulation of estradiol-17β-glucuronide, unlike those expressing human SLC22A10.

Jan 12, 2024 | nature.com | Ruchika Bajaj |Ilan E. Chemmama |Meghna Gupta |Andrej Sali |Yifan Cheng |Charles S. Craik

AbstractMultidrug resistance protein 4 (MRP4) is a broadly expressed ATP-binding cassette transporter that is unique among the MRP subfamily for transporting prostanoids, a group of signaling molecules derived from unsaturated fatty acids. To better understand the basis of the substrate selectivity of MRP4, we used cryogenic-electron microscopy to determine six structures of nanodisc-reconstituted MRP4 at various stages throughout its transport cycle.