Shuibing Chen

May 21, 2024 | nature.com | Hermany Munguba |Arianna Baggiolini |Conor Liston |Shuibing Chen |Gabriele Ciceri

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-023-02031-z, published online 2 January 2024. In Fig. 3h–k we recorded spontaneous excitatory postsynaptic currents (sEPSCs) in human PSC-derived cortical neurons at either –60 mV or at 0 mV, comparing their synaptic properties following GENtoniK versus DMSO treatment. We reported those currents as either “AMPAR”- or “NMDAR”-mediated, respectively.

Jan 17, 2024 | cell.com | Liuliu Yang |Manoj Nair |Oliver Harschnitz |Jiajun Zhu |Pengfei Wang |So Yeon Koo | +17 more

hPSC differentiation toward DA neuronsMidbrain dopaminergic neuron differentiation were performed using H9, MEL1 and SNCA different copy numbers hPSCs, which include NURR1: GFP hPSC. hPSCs were grown on VTN-N (Thermo Fisher Scientific)-coated 6-well plates in E8-essential medium. Cells were maintained at 37°C, 5% CO2.

Jan 1, 2024 | nature.com | Hermany Munguba |Arianna Baggiolini |Conor Liston |Shuibing Chen |Gabriele Ciceri

AbstractThe maturation of human pluripotent stem cell (hPSC)-derived neurons mimics the protracted timing of human brain development, extending over months to years for reaching adult-like function. Prolonged in vitro maturation presents a major challenge to stem cell-based applications in modeling and treating neurological disease.