Orit Rozenblatt-Rosen

Jul 15, 2024 | nature.com | Alexandra Schnell |Maryann Zhao |Elena Christian |Sarah Zaghouani |Vasundhara Singh |Anvita Singaraju | +6 more

AbstractInterleukin-17 (IL-17)-producing helper T (TH17) cells are heterogenous and consist of nonpathogenic TH17 (npTH17) cells that contribute to tissue homeostasis and pathogenic TH17 (pTH17) cells that mediate tissue inflammation. Here, we characterize regulatory pathways underlying TH17 heterogeneity and discover substantial differences in the chromatin landscape of npTH17 and pTH17 cells both in vitro and in vivo.

Apr 17, 2024 | nature.com | Jiarui Ding |John Garber |Grace Carter |Toni Delorey |Orit Rozenblatt-Rosen |Daniel Graham | +2 more

AbstractCoordinated cell interactions within the esophagus maintain homeostasis, and disruption can lead to eosinophilic esophagitis (EoE), a chronic inflammatory disease with poorly understood pathogenesis. We profile 421,312 individual cells from the esophageal mucosa of 7 healthy and 15 EoE participants, revealing 60 cell subsets and functional alterations in cell states, compositions, and interactions that highlight previously unclear features of EoE.

Aug 3, 2023 | nature.com | Ginevra Caratu |Orit Rozenblatt-Rosen |Wendy Luo |Christoph Muus |Fabiana M. Duarte |M. Ryan Corces | +19 more

AbstractSingle-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) has emerged as a powerful tool for dissecting regulatory landscapes and cellular heterogeneity. However, an exploration of systemic biases among scATAC-seq technologies has remained absent.

Jun 21, 2023 | nature.com | Yoon-Chul Kye |Elena Torlai Triglia |Alexandra Schnell |Toni Delorey |Kanishka Mohib |Christopher J. Giuliano | +11 more

AbstractThe role of B cells in anti-tumour immunity is still debated and, accordingly, immunotherapies have focused on targeting T and natural killer cells to inhibit tumour growth1,2. Here, using high-throughput flow cytometry as well as bulk and single-cell RNA-sequencing and B-cell-receptor-sequencing analysis of B cells temporally during B16F10 melanoma growth, we identified a subset of B cells that expands specifically in the draining lymph node over time in tumour-bearing mice.

Feb 16, 2023 | nature.com | Simon Mages |Inbal Avraham-Davidi |fei chen |Orit Rozenblatt-Rosen |Johanna Klughammer |Aviv Regev | +1 more

AbstractTransferring annotations of single-cell-, spatial- and multi-omics data is often challenging owing both to technical limitations, such as low spatial resolution or high dropout fraction, and to biological variations, such as continuous spectra of cell states.