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Oct 7, 2024 | 
nature.com | Brooke K Hayes |Marina Harper |Jessica Lewis |Joel Steele |David L. Steer |Ralf B. Schittenhelm | +1 more
AbstractThe type VI secretion system (T6SS) is a molecular machine utilised by many Gram-negative bacteria to deliver antibacterial toxins into adjacent cells. Here we present the structure of Tse15, a T6SS Rhs effector from the nosocomial pathogen Acinetobacter baumannii. Tse15 forms a triple layered β-cocoon Rhs domain with an N-terminal α-helical clade domain and an unfolded C-terminal toxin domain inside the Rhs cage.
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Sep 27, 2024 | 
nature.com | Yuqin Wu |Susanne Hille |Jared Johnson |Cheng Huang |Mohammad Rahbari |Suchira Gallage | +7 more
AbstractThe peptide hormone glucagon is a fundamental metabolic regulator that is also being considered as a pharmacotherapeutic option for obesity and type 2 diabetes. Despite this, we know very little regarding how glucagon exerts its pleiotropic metabolic actions. Given that the liver is a chief site of action, we performed in situ time-resolved liver phosphoproteomics to reveal glucagon signaling nodes.
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Sep 2, 2024 | 
nature.com | Elizabeth Reisman |Javier Botella |Ralf B. Schittenhelm |David Stroud |Cesare Granata |Georg Ramm | +1 more
AbstractAnalyses of mitochondrial adaptations in human skeletal muscle have mostly used whole-muscle samples, where results may be confounded by the presence of a mixture of type I and II muscle fibres. Using our adapted mass spectrometry-based proteomics workflow, we provide insights into fibre-specific mitochondrial differences in the human skeletal muscle of men before and after training.
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Apr 15, 2024 | 
nature.com | Pok Man Leung |Rhys Grinter |Eve Tudor-Matthew |Carlo R. Carere |Matthew Stott |Ralf B. Schittenhelm
AbstractDiverse aerobic bacteria use atmospheric hydrogen (H2) and carbon monoxide (CO) as energy sources to support growth and survival. Such trace gas oxidation is recognised as a globally significant process that serves as the main sink in the biogeochemical H2 cycle and sustains microbial biodiversity in oligotrophic ecosystems. However, it is unclear whether archaea can also use atmospheric H2.
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Jan 20, 2024 | 
onlinelibrary.wiley.com | Mee Chee Chong |Anup Shah |Monash Proteomics |Ralf B. Schittenhelm
1 INTRODUCTION Accumulating evidence shows that physical exercise contributes to overall health benefits, including enhanced cardiovascular fitness, elevated overall metabolism, improved immune competence, and the prevention of chronic diseases such as cardiovascular disease and cancer.1-3 Despite the impact of exercise on human health that is unequivocal, the exact mechanisms for how exercise promotes overall health remain poorly understood at the signalling level.
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Nov 17, 2023 | 
dx.doi.org | Liam M. Koehn |Joel Steele |Ralf B. Schittenhelm |Bradley Turner
Amyotrophic lateral sclerosis (ALS) is characterized by death and dysfunction of motor neurons that result in a rapidly progressing loss of motor function. While there are some data on alterations at the blood–brain barrier (BBB) in ALS and their potential impact on CNS trafficking of drugs, little is reported on the impact of this disease on the expression of drug-handling proteins in the small intestine and liver.
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Nov 14, 2023 | 
biorxiv.org | Travis Johnson |Ralf B. Schittenhelm |Matthew Piper |Joshua N Johnstone
AbstractMany mechanistic theories of ageing argue that a progressive failure of somatic maintenance, the use of energy and resources to prevent and repair damage to the cell, underpins ageing. To sustain somatic maintenance an organism must acquire dozens of essential nutrients from the diet, including essential amino acids (EAAs), which are physiologically limiting for many animals.
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Jun 15, 2023 | 
nature.com | Katie Ayers |Nicole A. Siddall |Liang Zhao |Josephine Bowles |Ginevra Zanni |Lydie Burglen | +6 more
Correction to: Nature Communications https://doi.org/10.1038/s41467-023-39040-0, published online 09 June 2023In this article, the author name Kenneth McElreavey was incorrectly written as Kenneth MacElreavey. The original article has been corrected.
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Jun 9, 2023 | 
nature.com | Katie Ayers |Liang Zhao |Josephine Bowles |Ginevra Zanni |Lydie Burglen |Ralf B. Schittenhelm | +6 more
AbstractSquamous cell carcinoma antigen recognized by T cells 3 (SART3) is an RNA-binding protein with numerous biological functions including recycling small nuclear RNAs to the spliceosome. Here, we identify recessive variants in SART3 in nine individuals presenting with intellectual disability, global developmental delay and a subset of brain anomalies, together with gonadal dysgenesis in 46,XY individuals.
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Mar 11, 2023 | 
nature.com | Pacman Szeto |Miles C Andrews |Ralf B. Schittenhelm
AbstractCellular heterogeneity in cancer is linked to disease progression and therapy response, although mechanisms regulating distinct cellular states within tumors are not well understood. We identified melanin pigment content as a major source of cellular heterogeneity in melanoma and compared RNAseq data from high-pigmented (HPCs) and low-pigmented melanoma cells (LPCs), suggesting EZH2 as a master regulator of these states.
