James S. Trimmer

Aug 4, 2024 | nature.com | Xiaomeng Han |Xiaotang Lu |Peter Li |Zudi Lin |Daniel Berger |Yuelong Wu | +6 more

AbstractMapping neuronal networks is a central focus in neuroscience. While volume electron microscopy (vEM) can reveal the fine structure of neuronal networks (connectomics), it does not provide molecular information to identify cell types or functions. We developed an approach that uses fluorescent single-chain variable fragments (scFvs) to perform multiplexed detergent-free immunolabeling and volumetric-correlated-light-and-electron-microscopy on the same sample.

Dec 1, 2023 | eneuro.org | Kristina D Micheva |Richard J. Weinberg |Stephen Smith |James S. Trimmer

connectivityelectron microscopynanoscaleproteomesynaptomeultrastructureSignificance StatementArray tomography (AT) is a powerful volume microscopy technique for high-dimensional analysis of complex protein populations in cells and organelles, including synapses. AT involves the use of ultrathin serial sections embedded in resin and subjected to multiple rounds of immunofluorescence antibody (Ab) labeling and imaging.

Nov 13, 2023 | nature.com | Collin Matsumoto |Declan Manning |Paula Rhana |Zhihui Fong |Nicholas C. Vierra |James S. Trimmer

AbstractIn arterial myocytes, the canonical function of voltage-gated CaV1.2 and KV2.1 channels is to induce myocyte contraction and relaxation through their responses to membrane depolarization, respectively. Paradoxically, KV2.1 also plays a sex-specific role by promoting the clustering and activity of CaV1.2 channels. However, the impact of KV2.1 protein organization on CaV1.2 function remains poorly understood.

Sep 27, 2023 | nature.com | James S. Trimmer

AbstractThe Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq) is a concerted effort to determine and make publicly available hybridoma-derived sequences of monoclonal antibodies (mAbs) valuable to neuroscience research. Over 30 years of research and development efforts including those at the UC Davis/NIH NeuroMab Facility have resulted in the generation of a large collection of mouse mAbs validated for neuroscience research.

Jul 28, 2023 | nature.com | Maria Ximena Casas |María Casas |Sergi Simó |James S. Trimmer |Rose E. Dixon

AbstractLysosomes communicate through cholesterol transfer at endoplasmic reticulum (ER) contact sites. At these sites, the Niemann Pick C1 cholesterol transporter (NPC1) facilitates the removal of cholesterol from lysosomes, which is then transferred to the ER for distribution to other cell membranes. Mutations in NPC1 result in cholesterol buildup within lysosomes, leading to Niemann-Pick Type C (NPC) disease, a progressive and fatal neurodegenerative disorder.