Karen Adelman

Jan 10, 2025 | biorxiv.org | Claudia Mimoso |Hanneke Vlaming |Karen Adelman |Ct Usa

AbstractThe eukaryotic genome is broadly transcribed by RNA polymerase II (RNAPII) to produce protein-coding messenger RNAs (mRNAs) and a repertoire of non-coding RNAs (ncRNAs). Whereas RNAPII is very processive during mRNA transcription, it terminates rapidly during synthesis of many ncRNAs, particularly those that arise opportunistically from accessible chromatin at gene promoters or enhancers.

Nov 3, 2024 | nature.com | Umut Berkay Altıntaş |Claudia Giambartolomei |Geoffrey Nelson |Seth Goldman |Karen Adelman |Matthew Freedman

AbstractAndrogen receptor (AR)-mediated transcription plays a critical role in development and prostate cancer growth. AR drives gene expression by binding to thousands of cis-regulatory elements (CRE) that loop to hundreds of target promoters. With multiple CREs interacting with a single promoter, it remains unclear how individual AR bound CREs contribute to gene expression.

Jan 28, 2024 | nature.com | Emily Kaye |Geoffrey Nelson |Huanyu Qiao |Karen Adelman |Kavyashree Basavaraju

AbstractMale germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations in gene expression during spermatogenesis associated with infertility. Here, we use steady-state, nascent and single-cell RNA sequencing strategies to comprehensively characterize gene expression across male germ cell populations, to dissect the mechanisms of gene control and provide new insights towards therapy.

Dec 17, 2023 | biorxiv.org | Tuo Zhang |Ryan J. Marina |Rab K Prinjha |Karen Adelman

AbstractExposure of unicellular or multicellular organisms to adverse environmental conditions, including nutrient deprivation, may induce a state of suspended animation or diapause. The diapause minimizes the organism's reliance on external energy sources and ensures survival. Among different forms of diapause, embryonic diapause, caused by a limited supply of nutrients to the growing embryos, is particularly challenging for the organism.

Mar 24, 2023 | cell.com | Claudia Mimoso |Karen Adelman

The expansion of introns within mammalian genomes poses a challenge for the production of full-length messenger RNAs (mRNAs), with increasing evidence that these long AT-rich sequences present obstacles to transcription. Here, we investigate RNA polymerase II (RNAPII) elongation at high resolution in mammalian cells and demonstrate that RNAPII transcribes faster across introns.