Journal of Biomedical Science

Jan 14, 2025 | jbiomedsci.biomedcentral.com | Sun Yat-sen

Human lung cancer cell A549, cervical cancer cell HeLa and SiHa, breast cancer MDA-MB-231 were commercially obtained from ATCC and maintained by our laboratory. We used DMEM for HeLa and SiHa cells, RPMI 1640 for MDA-MB-231 and A549 cells. Cells in the culture medium were supplemented with 10% FBS (Procell, China) and 100 U/mL penicillin/streptomycin (Beyotime, China) under an atmosphere of 5% CO2 at 37 °C.

Jan 8, 2025 | jbiomedsci.biomedcentral.com | La Trobe

Many tumour-promoting factors derived from CAFs have been identified in the past, positioning them as promising targets for therapeutic interventions. These factors can either directly interact with cancer cells to regulate tumour actions, or affect other stromal components like immune cells. Many clinical trials have been carried out to assess the efficacy of drugs targeting these CAF-derived factors (Fig. 4; Table 4). Drugs targeting CAF-derived factors that promote tumour development.

Jan 5, 2025 | jbiomedsci.biomedcentral.com | Yulong YU |Han Yin |Weiheng Zhao |Yuan Wang |Qianqian Yu |Xianglin Yuan | +2 more

The F. nucleatum strain (ATCC 25586) was obtained from the China General Microbiological Culture Collection Center and cultured anaerobically (MGC, AnaeroPack) in brain heart infusion (Landbridge, CM917B) broth medium at 37 °C. The human CRC cell lines (HT29, SW480) and the mouse CRC cell line CT26 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and preserved in liquid nitrogen.

Dec 23, 2024 | jbiomedsci.biomedcentral.com | Aviral Kumar |Juncheng Lyu |Mangala Hegde |Mohamed Abbas |Mohammed S Alqahtani |Ali J. Zarrabi | +6 more

Autophagy is an intricate, evolutionary conserved and agile process eliminating misfolded proteins, damaged or aged organelles and mutated proteins, that contains three types such as microautophagy, macroautophagy and chaperone-mediated autophagy, where the macroautophagy has received significant attention and thorough investigation [40].

Oct 5, 2024 | jbiomedsci.biomedcentral.com | Taoyuan City

In the 1980s, scientific exploration of extracellular vesicles (EVs) began, initially focusing on reticulocyte maturation, which revealed EVs containing transferrin receptors were crucial for modulating reticulocyte maturation [1]. Subsequently, these EVs were identified as “exosomes”, originating from fusion of multivesicular bodies (MVBs), displaying phospholipid-bilayer-enclosed spherical structures and carrying diverse cargos [2, 3].