Jason D. Buenrostro

3 weeks ago | nature.com | Chiara Herzog |Kejun Ying |Raghav Sehgal |Waylon J. Hastings |Alexander Tyshkovskiy |Sara Hägg | +19 more

On 1–2 November 2024, the annual Biomarkers of Aging conference welcomed academic and industry scientists, and partners from governmental and nongovernmental organizations, to Harvard Medical School in Boston, USA, to discuss new insights into measuring and monitoring human aging, with the aim of clinical translation. In this Meeting Report, we summarize the conference and offer potential future directions for the Biomarkers of Aging Consortium and the longevity science community at large.

Jul 15, 2024 | nature.com | Alexandra Schnell |Maryann Zhao |Elena Christian |Sarah Zaghouani |Vasundhara Singh |Anvita Singaraju | +6 more

AbstractInterleukin-17 (IL-17)-producing helper T (TH17) cells are heterogenous and consist of nonpathogenic TH17 (npTH17) cells that contribute to tissue homeostasis and pathogenic TH17 (pTH17) cells that mediate tissue inflammation. Here, we characterize regulatory pathways underlying TH17 heterogeneity and discover substantial differences in the chromatin landscape of npTH17 and pTH17 cells both in vitro and in vivo.

May 20, 2024 | nature.com | Giulia Schiroli |Fabiana M. Duarte |Andrew Earl |Jason D. Buenrostro

AbstractHematopoietic stem cell (HSC) mutations can result in clonal hematopoiesis (CH) with heterogeneous clinical outcomes. Here, we investigate how the cell state preceding Tet2 mutation impacts the pre-malignant phenotype. Using an inducible system for clonal analysis of myeloid progenitors, we find that the epigenetic features of clones at similar differentiation status are highly heterogeneous and functionally respond differently to Tet2 mutation.

Aug 31, 2023 | nature.com | Caleb A Lareau |Leif S. Ludwig |Christoph Muus |Gad Getz |fei chen |Jason D. Buenrostro | +3 more

Correction to: Nature Biotechnology https://doi.org/10.1038/s41587-020-0645-6. Published online 12 August 2020. In the version of this article initially published, due to a coding error, cell clonotypes and allele frequencies were permuted, impacting the visualization of panels in Fig. 6i, j, k, and o. Corrected panels appear as Fig. 1 below, and the software and documentation listed in the Code availability section have been updated.

Aug 3, 2023 | nature.com | Ginevra Caratu |Orit Rozenblatt-Rosen |Wendy Luo |Christoph Muus |Fabiana M. Duarte |M. Ryan Corces | +19 more

AbstractSingle-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) has emerged as a powerful tool for dissecting regulatory landscapes and cellular heterogeneity. However, an exploration of systemic biases among scATAC-seq technologies has remained absent.