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Jan 14, 2025 | 01041-4?rss=yes&sz=64)
cell.com | Xiang Zheng |Andreas Mund |Matthias Mann
Keywords multiplexed imaging mass spectrometry deep visual proteomics spatial proteomics spatial medicine tumor microenvironment cancer immunotherapy tumor-infiltrating lymphocytes Introduction The tumor microenvironment (TME) is a complex ecosystem where cancer cells dynamically interact with diverse non-malignant cells, including immune cells, fibroblasts, and endothelial cells, as well as the extracellular matrix.1 These intricate interactions shape cancer progression and therapy...
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Dec 5, 2024 | 
nature.com | Thierry M. Nordmann |Andreas Mund |Matthias Mann
Spatial mass spectrometry (MS)-proteomics is a rapidly evolving technology, particularly in the form of Deep Visual Proteomics (DVP), which allows the study of single cells directly in their native environment. We believe that this approach will reshape our understanding of tissue biology and redefine fundamental concepts in cell biology, tissue physiology and ultimately human health and disease.
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Nov 1, 2024 | 
biorxiv.org | Ashok Kumar Jayavelu |Matthias Mann |Natalie Krahmer |Paul T. Pfluger
AbstractReversible post-translational modification (PTMs) is a fundamental mechanism of cellular signal transduction. In vivo and ex vivo studies to profile PTMs have greatly advanced our understanding of the complexities of cellular signaling. However, apart from the most commonly studied PTMs, large-scale analysis is still very challenging, limiting our understanding of various cellular processes.
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Aug 27, 2024 | 
nature.com | Abdolhossein Zare |Saeede Salehi |Jakob M. Bader |Utz Fischer |Alexander Veh |Panagiota Arampatzi | +2 more
AbstractMotoneurons critically depend on precise spatial and temporal control of translation for axon growth and the establishment and maintenance of neuromuscular connections. While defects in local translation have been implicated in the pathogenesis of motoneuron disorders, little is known about the mechanisms regulating axonal protein synthesis.
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Aug 22, 2024 | 00837-7&sz=64)
cell.com | Yvonne Chen |Charles Evavold |Matthias Mann |Emily Davenport
In vitro human cell-based models: What can they do and what are their limitations?
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Jun 25, 2024 | 
nature.com | Henry E. Webel |Lili Niu |Marie Locard-Paulet |Matthias Mann
AbstractImputation techniques provide means to replace missing measurements with a value and are used in almost all downstream analysis of mass spectrometry (MS) based proteomics data using label-free quantification (LFQ). Here we demonstrate how collaborative filtering, denoising autoencoders, and variational autoencoders can impute missing values in the context of LFQ at different levels.
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Jun 13, 2024 | 
biorxiv.org | Matthias Mann |Xiang Zheng |Lars Pedersen |Michael Bzorek
AbstractCoexistence of two cancer types in the same organ presents challenges for clinical decision-making, calling for personalized treatment strategies. Deep Visual Proteomics (DVP) combines AI driven single cell type analysis with laser microdissection and ultrasensitive mass spectrometry. In a composite case of classical Hodgkin lymphoma (cHL) and small lymphocytic lymphoma (SLL) in a single patient, we investigated the potential of DVP to inform precision oncology.
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May 23, 2024 | 
biorxiv.org | Xiang Zheng |Andreas Mund |Matthias Mann
AbstractDeciphering the intricate tumor–immune interactions within the microenvironment is crucial for advancing cancer immunotherapy. Here, we developed a novel approach integrating highly multiplexed imaging, laser microdissection, and deep visual proteomics (DVP) to spatially profile the proteomes of 21 distinct cell populations in human colorectal and tonsil cancers with high sensitivity.
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May 16, 2024 | 00329-0?rss=yes&sz=64)
cell.com | Sara Sepic |Annette Gross |Helmholtz Zentrum München |Research Unit-Signaling |Kamyar Hadian |Peter Murray | +5 more
Highlights • Supramolecular WDR26-CTLH E3 complex ubiquitylates oligomeric metabolic enzyme NMNAT1 • WDR26-CTLH E3 recognizes internal basic degron of NMNAT1 through WDR26 β-propellers • Degron mimicry of YPEL5’s N terminus inhibits NMNAT1 targeting by WDR26-CTLH E3 • YPEL5-WDR26-CTLH E3 modulates cellular NMNAT1 amounts affecting its metabolic function Summary The yeast glucose-induced degradation-deficient (GID) E3 ubiquitin ligase forms a suite of complexes with interchangeable receptors...
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Apr 29, 2024 | 
nature.com | Florian A. Rosenberger |Arnaud Mourier |Diana Rubalcava-Gracia |Polyxeni Papadea |Olov Andersson |Raffaella Di Lucrezia | +6 more
AbstractThe oxidative phosphorylation system1 in mammalian mitochondria plays a key role in transducing energy from ingested nutrients2. Mitochondrial metabolism is dynamic and can be reprogrammed to support both catabolic and anabolic reactions, depending on physiological demands or disease states. Rewiring of mitochondrial metabolism is intricately linked to metabolic diseases and promotes tumour growth3,4,5.
